Scientist, Multi-Specifics Expression

AbbVie's mission is to discover and deliver innovative medicines and solutions that solve serious health issues today and address the medical challenges of tomorrow. We strive to have a remarkable impact on people's lives across several key therapeutic areas – immunology, oncology, neuroscience, and eye care – and products and services in our Allergan Aesthetics portfolio. For more information about AbbVie, please visit us at www.abbvie.com. Follow @abbvie on X, Facebook, Instagram, YouTube, LinkedIn and Tik Tok.

AbbVie Biotherapeutics and Genetic Medicine is an integrated, global discovery organization responsible for biotherapeutics drug research across disease areas including immunology, oncology, neuroscience, and eye care.  Our scope includes the discovery of innovative biologic medicines from the early exploratory stage and their support through clinical development.  We are growing a world-class team of scientists and leaders who will drive the discovery and advancement of innovative biologic-based therapies.  Careers in AbbVie Biotherapeutics and Genetic Medicine provide unique opportunities to work with cross-functional teams that drive exceptional science and deliver innovative medicines to patients.  We are looking for diverse candidates with strong scientific and leadership experience to join the team and can offer exciting and rewarding career opportunities for these candidates.

The Biotherapeutics Production group at AbbVie is seeking an experienced bispecifics/multi-specifics cell line and protein expression scientist to join a fast paced, high throughput, large scale mammalian expression group. The Biotherapeutics mammalian expression group supports medium to large scale (1 to 100L) expression of bispecific/multi-specific biologics in support of AbbVie’s worldwide drug discovery efforts.  

Key Responsibilities:

• Expression of bispecifics and other novel multi-specific biologics formats to support therapeutic area drug discovery projects.
• Transient and stable CHO expression vector design (including complex 3-chain and 4-chain multi-specific molecules),
• Generation of stable CHO cell lines using 2 or more vectors and multiple selection strategies to optimize chain pairing of 3- and 4-chain molecules.
• Transient protein expression of 3- and 4-chain molecules in CHO cells, and process optimization to determine optimal chain ratio.
• Maintenance of stable CHO pools and cell lines- including thaw, culture, expression and cryopreservation.
• Process development and optimization for batch and fed-batch Wave bioreactor and shake flask expressions of transient and stable CHO cell lines expressing multi-specifics.
• Purification and biophysical characterization of analytical scale expression samples, using analytical HPLC to evaluate titer, % monomer, and chain pairing efficiency.
• Work independently on the design, execution, and interpretation of experimental results.
• Analyze, document, and report data concisely and rigorously.  

Qualifications:

• Bachelors or Masters in biology, chemistry, biochemistry or related field with typically 5-7+ (BS) or 2-5+ (MS) years of experience.
• Hands-on experience with cell culture and transient protein expression of bi-specifics and multi-specifics in CHO cells. 
• Prior experience in transient and stable vector design and generation of stable CHO cell lines expressing complex 3- and 4-chain multi-specific molecules.
• Prior experience with analytical characterization of multi-specifics desirable.
• Prior experience in mammalian expression using Wave bioreactors desirable. 
• Experience in process development and process optimization.
• Demonstrated proficiency in overcoming problems (i.e. poor chain pairing, low expression, aggregation, glycosylation) with challenging therapeutic multi-specific proteins desirable.

Position will be filled at level commensurate with extent of education, experience, and accomplishment - Scientist I or Scientist II. 

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law: ​

​

• The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.​

• We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.​

• This job is eligible to participate in our short-term incentive programs. ​

​

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employee remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company’s sole and absolute discretion, consistent with applicable law. ​

​

AbbVie is an equal opportunity employer and is committed to operating with integrity, driving innovation, transforming lives, serving our community and embracing diversity and inclusion.  It is AbbVie’s policy to employ qualified persons of the greatest ability without discrimination against any employee or applicant for employment because of race, color, religion, national origin, age, sex (including pregnancy), physical or mental disability, medical condition, genetic information, gender identity or expression, sexual orientation, marital status, status as a protected veteran, or any other legally protected group status.

US & Puerto Rico only - to learn more, visit https://www.abbvie.com/join-us/equal-employment-opportunity-employer.html 

US & Puerto Rico applicants seeking a reasonable accommodation, click here to learn more:

https://www.abbvie.com/join-us/reasonable-accommodations.html